August 23, 2011  |  Education, Flow Cytometry

Future Proofing Your Experiments and Files: The Importance of Annotation

Ever find yourself staring at a folder of FCS files and thinking, “Wait, now which tubes did I add PMA to, how much did I add, and which samples were these again?”

Jonathan from Cytobank/Stanford recommends what he calls “future proofing” in order to avoid this problem. He explained this approach during a CYTO 2011 Pre-Congress course in his talk titled “Flood Cytometry: Embracing Single Cell Systems Biology (and coping with large cytometry experiments).” In that talk, he outlined four easy steps that are useful for experiments of all sizes.

When collecting on the cytometer:

  1. Tag your FCS files with key experiment details (e.g. “Patient-J01 IL-2 15m”)
  2. Label the channels you are measuring (before collecting data)
  3. Make sure scales and compensations work (before collecting data)
  4. Encode clinical sample IDs (don’t use HIPAA sensitive information)
Click the image to download as a PPT slide

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May 11, 2011  |  Announcements

Upcoming Meetings: IMMUNOLOGY 2011 and CYTO 2011

We’re excited to be attending both IMMUNOLOGY 2011 and CYTO 2011 in the next couple weeks. Come say hello and tell us how you’re using Cytobank to share and analyze your flow data.

May 13-17, 2011
IMMUNOLOGY 2011
98th Annual Meeting of the American Association of Immunologists
Moscone Center, San Francisco, CA
Booth 307

May 21-25, 2011
CYTO 2011
XXVI Congress of the International Society for Advancement of Cytometry (ISAC)
Baltimore Convention Center, Baltimore, MD
Booth 322

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May 6, 2011  |  Flow Cytometry

Mass Cytometry: Vaporizing Cells in the Name of Science

Mass cytometry, a technique developed by DVS Sciences, now a Fluidigm Company represents a revolutionary spin on classic fluorescence-based flow cytometry. Instead of using antibodies tagged with fluorophores (in which spectral overlap quickly limits the number of parameters available for simultaneous detection), mass cytometry relies on antibodies tagged with transition element isotopes. Antibody-bound cells are vaporized, ionized, and analyzed on a mass spectrometer.

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April 9, 2011  |  Education, Flow Cytometry

Teaching Phospho-Flow… in France

Dataset #5002: Timecourse LACI 2011

This January, Jonathan and Chris from Cytobank traveled to Marseilles, France to help lead a course as part of the Luminy Advanced Course in Immunology (LACI). LACI is organized as a satellite meeting to the Immunology and Metabolism meeting and organized by the Centre d’Immunlogie de Marseille-Luminy (CIML) and the European Molecular Biology Organization (EMBO).

The ‘Cell Signaling’ course at LACI was taught by local instructors Nathalie Auphan-Anezin and Pierre Grenot, both of CIML, and Jonathan and Chris. The course led course participants through staining, collection, upload, and analysis of a phospho-flow experiment. We’ve briefly described the experiment here, made a version of the dataset public along with the original course protocol, and prepared a tutorial (part 1 and part 2) to lead you through Cytobank analysis of the course data.

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April 6, 2011  |  Announcements

Upcoming Meetings: May 2011

Two big meetings are on our calendar for May. Are you attending either of these? If so, come say hello and tell us how you use Cytobank to share and analyze your flow data.

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April 5, 2011  |  Announcements

That Funny Looking Black-and-White Box

You may have noticed a little black-and-white box in the top right corner of your printed Illustrations. This box is a two-dimensional barcode called a QR code.

The QR code for every printed Illustration from Cytobank encodes:

  • Name of Experiment
  • Name of Illustration
  • Link to Illustration (print view)

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