July 21, 2014  |  User Stories  |  By  |  0 Comments

Cytobank User Stories: Marianne Enger

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of fluorescence and mass cytometry.

This time we interview Marianna Enger, the flow cytometry core facility manager at University of Bergen.

What are you excited about in science? What is your scientific vision?
Marianna Enger

Marianna Enger
Flow Cytometry Core Facility Manager at University of Bergen

My vision as a flow cytometry core facility manager is to make flow cytometry available and affordable to anyone interested. The ambition is to provide research groups with the specialized resources and the expertise they need to produce the best possible scientific output in a cost effective way.

Cell signaling pathways interact with one another to form networks and my special interest is in hematopietic stem cells and signaling pathways. Flow cytometry is great for exploring signaling networks which I find very challenging and exciting and my wishes for the future include a CyTOF. My focus for the last two years has been on normal hematopoietic cellular responses and their potential disregulation in disease.

What do you study / what is your field?
First of all, I am a flow cytometry core facility person, but I also have the opportunity to be involved in a project and work in the lab, which is great. Flow analysis on hematopoietic stem cells, signal transduction, cell sorting for further analysis, training users, and arranging meetings and courses are some of my tasks. In all of this, Cytobank fits very well as a very user-friendly software for complex datasets.
What do you use flow cytometry for?
I guide and train users all the time both for sorting and analysis. I often feel like a “control-freak” telling people to include more control samples in their experiments and to titrate their antibodies! But there’s no way to get around this if you want to get the best result out of your data. A great experiment deserves the best controls!
What do you do for fun?
My family and I enjoy many holidays at our cabin in the Norwegian mountains both summer and winter for hiking or cross country skiing, but this summer we will go to Malaysia on vacation. We are so looking forward to the trip! After the vacation we will welcome a puppy to our house, so I guess all spare time will be occupied for a while.
What’s your favorite thing about Cytobank?
What I really love about Cytobank is that it is available and affordable for everyone! Cytobank is intuitive and both free and easy to use and this is important for many of my users. Your data/FCS files are accessible from every PC/Mac you are using – and you can easily share your data with your supervisor or collaborators, which is very important. I also like that I can demonstrate complex data sets with diverse options of data display with statistics without great effort. In a training session with my users, it´s easy to demonstrate Cytobank on their own PC/Mac. We are not dependent on a dongle or a license.
What are some of your favorite papers?
This paper gets better for every time I read it!

1. Single-cell phospho-specific flow cytometric analysis demonstrates biochemical and functional heterogeneity in human hematopoietic stem and progenitor compartments.  Gibbs KD Jr. et al. Blood (2011) 117(16):4226-33. [Journal]

A great way to start your flow cytometer multicolor experiment:

2. A practical approach to multicolor flow cytometry for immunophenotyping. Baumgarth N and Roederer M. J Immunol Methods (2000) 243(1-2):77-97. [Journal]

My first phospho-flow paper:

3. Single cell profiling of potentiated phospho-protein networks in cancer cells. Irish JM et al. Cell (2004) 118(2):217-28. [Journal]

Send us feedback and let us know who you’d like to hear from (including yourself)!

Interview conducted by Cytobank Product Manager Angela Landrigan.