June 17, 2011  |  Announcements, Flow Cytometry  |  By  |  0 Comments

BD FACSelect™: Resources to Optimize Your Flow Experiments

Designing a successful flow experiment – selecting compatible reagents and optimizing your protocol – can be challenging and time-consuming. And yet, as we all know, a well-designed experiment is critical to the collection of high-quality flow data.

What do we think about when designing flow experiments?

  • What buffers should I use when probing intracellular targets?
  • Which surface antibodies work well on my sample and with my buffers?
  • What is the best concentration for my antibody?
  • Are there alternative protocols that work better with my samples?

We are excited to announce the arrival of two resources that will help you answer those questions and streamline your reagent selection process. BD Biosciences has released the FACSelect™ series, consisting of a Multicolor Panel Designer and a Buffer Compatibility Resource.

Cytobank collaborated closely with BD to produce the Buffer Compatibility Resource, which makes primary data from BD’s extensive antibody testing available to you. Through an interactive page listing key details of tested antibodies and fluorochromes, you can click “View” to consult a detailed datasheet (powered by Cytobank Reports) and then click again to interact with the raw data in Cytobank.

How does this help me?

Currently, the Buffer Compatibility Resource offers extensive testing data on over 50 antibodies available in BD’s catalog. BD scientists stained human and mouse primary samples, as well as various cell lines. Samples were also permeabilized with one of four buffers. Datasheets for each antibody are organized by fluorochrome and permeabilization buffer so that you can quickly assess the staining efficacy of the various conjugates in each of the buffers.

Both intracellular and surface antibodies have been tested. Thus, when designing phospho-flow experiments, the Buffer Compatibility Resource enables you to assess which reagent and buffer combination will give you optimal signal. Surface antibodies were also run at different titrations, providing you with valuable data on how to get the best staining resolution.

Finally, BD has also made available to you all of the experimental protocols that their scientists used to stain these samples and test these antibodies. Standard and alternative protocols can be accessed in the top right corner of the main Buffer Compatibility Resource page, and additional protocols are accessible through the link on the bottom of the box. Each datasheet also provides any necessary details on stimulation conditions or caveats that are specific to each reagent or sample.

A quick guide to the Buffer Compatibility Resource

The interface allows you to quickly search by keywords, filter by fluorochrome or laser, and sort search results by clicking on column headers. Clicking “View” brings you to a detailed datasheet with plots organized by fluorochomes and permeabilization buffers. Reagents for each datasheet are listed in the top right corner, along with a link to the BD catalog so you can quickly access the reagents you need.

Beneath each set of data plots, you will find a link to “View in Cytobank.” Clicking on this link will prompt you to log in to Cytobank, where you can interact with the data. You can also clone the experiment files to obtain your own copy of the data and begin gating and analyzing the data yourself.

Don’t have an account on Cytobank? Click here to register (for free).

Questions or comments? Don’t see your favorite antibody? Email us!

– The Cytobank Team