Posts tagged ‘science’

Cytobank User Stories: Sarah Tasian, M.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Sarah Tasian, M.D., Attending Physician and Instructor in the Division of Oncology at the Children’s Hospital of Philadelphia. She is part of the Grupp lab and co-mentored by Martin Carroll, M.D. Her recent publications include her work studying STAT5 and PI3K/mTOR signaling in B-precursor acute lymphoblastic leukemia cells (ALL) and studying the effects of JAK and mTOR inhibition in B-precursor ALL mouse xenografts.

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What are you excited about in science? What is your scientific vision?
Sarah Tasian, M.D. Children's Hospital of Philadelphia

Sarah Tasian, M.D. – Children’s Hospital of Philadelphia

With modern genomic and proteomic technologies, we are truly embarking upon the era of personalized medicine!
My long-term career goal as a translational physician-scientist in pediatric oncology is to develop better therapies, improve cure rates, and minimize toxicities for children with high-risk leukemias. In the laboratory, I study perturbations of signal transduction networks in genetic subtypes of pediatric B-precursor acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML) with the goal of identification of relevant targets for novel targeted therapies. I use specialized immunocompromised murine xenograft models of childhood ALL and AML for the preclinical testing of relevant signal transduction inhibitor (STIs) and of newer chimeric antigen receptor (CAR)-based T cell immunotherapy. I use phosphoflow cytometry to study signal transduction in primary patient leukemia samples and to measure molecular responses to STIs in vitro and in vivo. I am also involved in the testing of STIs in children with relapsed leukemias via Phase I clinical trials through the Children’s Oncology Group. Successful development of these laboratory and clinical research strategies will ultimately allow me to lead a translational research program in developmental therapeutics for children with clinically high-risk leukemias. (more…)

January 28, 2013 at 6:59 pm Leave a comment

Nolan Lab Signaling-Based Cytometry Resource

As part of educational and community outreach efforts in flow cytometry, the Nolan Lab at Stanford University has worked with the Cytobank team to launch a Signaling-Based (Fluorescence & Mass) Cytometry Resource. The Nolan lab has authored several key publications in flow cytometry on subjects including phosphoflow cytometry, barcoding, mass cytometry, and SPADE. Visit this Cytobank-powered resource to find experiment protocols for fluorescence and mass cytometry, Cytobank Reports, and Publications.

Experiment Protocols

The Experiment Protocols section of the resource links to protocols for fluorescence and mass cytometry, including those relevant to stimulations, barcoding, fix/perm methods, and titrating antibodies.

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July 31, 2012 at 5:10 pm Leave a comment

Cytobank User Stories: Greg Behbehani, Ph.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Greg Behbehani, Ph.D., Postdoctoral Fellow in the Nolan Lab, affiliated with the Baxter Laboratory of Stem Cell Biology and the Departments of Hematology and Oncology at Stanford University School of Medicine. Greg’s recent publications include his work developing cell cycle analysis for mass cytometry using the human hematopoietic hierarchy as a model system and is featured on the cover of Cytometry this month. You can interact directly with these data via Greg’s Cytobank Report, which can also be found on the Nolan Lab Signaling-Based (Fluorescence & Mass) Cytometry Resource.

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What are you excited about in science? What is your scientific vision?

Greg Behbehani, Ph.D. Postdoctoral Fellow, Nolan Lab, Stanford University

My research is focused on understanding how human cancer cells grow and die, specifically in response to chemotherapy treatments.  I’m excited about the new possibilities that have been opened up in systems biology.  Ideally, I would like to design experiments to test some of the hypotheses that have been advanced regarding how cancer cell populations become resistant to chemotherapy using primary cell populations and analyzing responses across all of the different cancer cell sub-populations.  While these types of experiments have been tried before, I think we now have the technologies that might allow us to be successful at using these techniques to predict chemotherapy responses.
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July 30, 2012 at 11:44 am Leave a comment

Cytobank User Stories: Harris Fienberg

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Harris Fienberg, a Ph.D. candidate in the Nolan Lab at Stanford University. Harris’s most recent publication features his work developing a cell viability detection protocol for mass cytometry using a platinum-based reagent. You can view and analyze the data firsthand via his Cytobank Report.

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What are you excited about in science? What is your scientific vision?

Harris Fienberg – Graduate Student, Nolan Lab, Stanford University

Over the last 30 years we’ve gained an extraordinary understanding of the molecular components that make up cellular signaling cascades. However, we’re just beginning to understand how the various components of the cell work together to integrate signals and relay these signals to form phenotypic outcomes. I’m interested in gaining a more holistic understanding of cellular communication. In the future I believe that specific proteins will be seen less in the context of certain signaling pathways and more as words that the cell uses to make a message. I think that reaching this more comprehensive understanding of cellular signaling will be more about about integrating data with smart data analytics than gaining more and more “-omics” style data sets. (more…)

May 30, 2012 at 9:05 am 1 comment

Cytobank User Stories: Joshua Brody, M.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Joshua Brody, M.D., Director of the Lymphoma Immunotherapy Program at Mount Sinai School of Medicine. Joshua’s recent publications include his studies showing that in situ vaccination with a TLR9 agonist induces systemic anti-lymphoma clinical responses, as well as his studies using immunotransplant to preferrentially expand T-effector cells to cure large lymphoma tumors.

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What are you excited about in science? What is your scientific vision?

Joshua Brody, M.D. Director of Lymphoma Immunotherapy – Mount Sinai

I’m excited about the fact that FINALLY we are really learning how to use the immune system to make cancers shrink and let patients live longer.  For years, tumor immunologists and ‘immunotherapists’ have thought: ”This should work.  We should be able to make this work!”, but every year our understanding of the complexity of the immune system has become clearer.  Finally, that understanding is being translated into therapies that help patients with cancer.  We have seen it with melanoma and prostate cancer in the past 2 years and we clearly see even more powerful therapies on the near-horizon.
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April 18, 2012 at 11:05 am Leave a comment

Cytobank User Stories: Ernesto Diaz-Flores, Ph.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Ernesto Diaz-Flores, Ph.D., a postdoctoral fellow in Mignon Loh’s lab and previously in Kevin Shannon’s lab, both located at UCSF. Ernesto’s recent publications include his contributions to studies of p53 loss in acute myeloid leukemia, STAT5 activation in myeloid malignancies, and K-Ras(G12D) expression in primary hematopoietic stem/progenitor cells.

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What are you excited about in science? What is your scientific vision?

Ernesto Diaz-Flores, Ph.D. Postdoctoral Fellow – Shannon Lab UCSF


Science is a very exciting field because every new question represents a new challenge. As a scientist, my biggest contribution would be to tease out the biochemical mechanisms leading to leukemia and use that information to predict therapeutic responses prior to subjecting the patient to the treatment.
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March 13, 2012 at 2:36 pm Leave a comment

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