Posts tagged ‘gating hierarchy’
On Cytobank, you can download the Illustrations you’ve built with our Print View and PDF tools. These are useful for generating figures for publications, printing for your lab notebook, and keeping a local copy of your Illustrations.
Our latest additions to this functionality include the ability for users to change the page size of PDFs such that large illustrations are no longer truncated horizontally.
Here is an overview of the changes:
Page formatting options
You can now select among a range of paper size options for PDFs, including three fixed-size options that constrain the page dimensions if you’re looking to print your plots (Letter, A4, and Poster) and two auto-fit options that scale the width and height of the PDF to exactly fit your document dimensions. The difference between the two auto-fit options has to do with limitations imposed by Adobe Acrobat Reader software, which will only open documents that do not exceed 200 inches in either dimension. So, if you plan on generating very large arrays of plots and want to use Adobe Acrobat Reader to view the PDF, you’ll need to select the “Very Large” auto-fit option, which inserts page breaks every 200 inches. If you do not want page breaks in your PDF and can use alternate software such as Adobe Illustrator to open your PDF, then choose the “Infinite” auto-fit option. With every page formatting option, if you choose a plot size and type combination that exceeds the width of the page format you have selected, an orange warning box will appear on the PDF generation page asking you to select a format with larger dimensions or to alter your plot size.
Dataset #8414: Human Cord Blood – HSC isolation
Hematopoietic Stem Cells (HSCs) give rise to all blood lineages and are capable of self-renewal. Clinically, HSC transplantation is under investigation for the treatment of diseases of the blood and bone marrow, including cancer, where a patient’s blood cells are wiped out and replaced with healthy cells that arise from transplanted donor HSCs. Transplant studies in mice have shown that only a few of these cells are necessary to repopulate the entire hematopoietic system.
Human umbilical cord blood is a rich source of stem cells, including HSCs. However, a variety of other cell types populate cord blood and must be removed from HSC preparations used for transplantation. Multipotent progenitor cells (MPPs) are one such population. Derived from HSCs, MPPs give rise to multiple lineages and are present in significant quantities in cord blood, though they are limited in their capacity for self-renewal. Purification of HSCs can be achieved by staining and running cord blood through a FACS sorter and isolating cells with a Lin-CD34+CD38-CD90+CD45RA- surface signature (as defined by Park, Majeti, and Weissman). MPPs can be quantified or isolated by their Lin-CD34+CD38-CD90-CD45RA- signature.
If you would like to try your hand at analyzing HSC enrichment data on Cytobank, we have made available an HSC dataset provided to us by scientists at BD Biosciences. You can find a tutorial to guide your analysis on our documentation site. (more…)