Posts tagged ‘data analysis’
You may have heard about Fluorescent Cell Barcoding, a flow cytometry technique that allows researchers to answer a larger number of questions with the same amount of antibody, as compared to standard flow cytometry experiments [1,2]. We’ve prepared a few resources to help you learn about, perform, and analyze barcoding experiments.
How does barcoding work? In the barcoding step, samples treated under different stimulation conditions are labeled with concentrations of dye that increase at a defined interval. The use of this dye to barcode effectively means that one cytometer channel is taken up for this code. The distinctly stimulated and labeled samples are then combined into one tube and stained with antibodies against targets of interest. This single tube is then run on a flow cytometer and data are collected for analysis. The most common approach is to barcode different stimulation conditions; however, barcoding can be applied to any distinct populations, such as patient samples or different time points of a stimulation condition.
SPADE analysis will be available through Cytobank soon. Do you want to make SPADE trees using your own fluorescence or mass cytometry data? Sign up for our newsletter or subscribe to our blog to get the latest news about when we will start to make SPADE available to our users.
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At Cytobank, we do cytometry in the “cloud”. What does that mean and how can that help you?
- Surviving the Data deluge
- Clarity, Access, and Collaboration
- Security and Back-up
Seeing the words “internet” and “experiment data” in the same sentence might give you pause to wonder, “Are my data safe on Cytobank?”
The answer is a resounding “Yes!”
From the minute you upload your data to Cytobank, you control the level of access that other users have to your data. By default, any data you upload are only visible to you.
Let’s just say it: compensation is a pain. Unfortunately, it’s a necessary pain if we want to accurately interpret our flow data and draw meaningful biological conclusions.
As a brief reminder of why we need to care about compensation, let’s examine the following emission spectra. We used the BD Fluorescence Spectrum Viewer; the Fluorescence SpectraViewer by Invitrogen/Life Technologies is also useful.