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December 14, 2010  |  Cytobank, Education, Flow Cytometry

Biochemistry at the Single Cell Level

Dataset #811: 090902-PBMC stim with IL6, IL10, LPS (Public Version)

Western blots are great. That is, unless you’re studying signal transduction in a mixed population of primary cells. The amount of material necessary for many biochemical techniques is usually not practical when working with patient samples or rare cell populations.

Flow cytometry to the rescue! Phospho-flow tracks biochemistry at the single cell level. Simultaneous assessment of cell surface markers and signaling events for individual cells enable biologists to profile disease samples or monitor the effects of drugs in clinical samples. More »

November 18, 2010  |  Cytobank

View Through a Heatmap: Have Your Cake and Eat It Too

Dataset #310: Phospho-Flow Cytokine Titration

Q: I want a big picture view of my flow cytometry experiment so that I can see how all of my samples are behaving under different conditions. But I also want to see the underlying single cell details because subsets of cells in these samples may be responding differently. Is this possible?

A: Absolutely. You can have your cake and eat it too by using a built-in feature on Cytobank to view-through a heatmap representation of your data. More »

November 17, 2010  |  Cytobank, Flow Cytometry

Take the U937 Challenge!

Dataset #61: Welcome to Cytobank – U937 dataset

So you want to do phospho-flow? Take the U937 challenge!

What is phospho-flow? It’s a specialized form of flow cytometry that enables biologists to probe signaling activity inside the cell. Flow cytometry has been widely used to analyze cell populations by probing the proteins that are outside on the cell surface. Phospho-flow cytometry includes a few extra steps – stimulation, fixation, and permeabilization – that enable researchers to also probe the transient phosphorylation state of intracellular signaling proteins. More »

October 14, 2010  |  Cytobank

The Cytobank Story

In the beginning, we wanted software that would give us a “big picture” view of our flow cytometry experiments.  We initially created a software tool to make heatmaps using flow cytometry files.  However, as we shared analyses with each other, we realized that this big picture view wasn’t enough.  We also needed to explore the single cell data to derive deeper meaning from our flow cytometry experiments.  In short, we wanted to have it both ways: we needed dynamic summaries of experiment results linked to the underlying single cell data, so that rare cell subsets and heterogeneous populations were not overlooked. More »

October 4, 2010  |  Announcements

More Articles Coming Soon

We’ve been working hard to improve Cytobank, but we’ll have more articles and updates coming soon. Subscribe to our blog and make sure you don’t miss a post! You can also stay connected by following us on Twitter (@cytobank).

Questions, comments, suggestions? We want to hear from you. Leave a comment or e-mail us at info@cytobank.org.

– Stephanie

April 23, 2010  |  Announcements

Welcome to the Cytobank Blog

Here’s a quick post welcoming everyone to our brand new blog.  One of the things you can expect to see here are posts about our software releases, which will provide a list of new features and bug fixes that have just been launched to our production servers.  We’re also going to try to have discussions about various aspects of cytometry and ideally even some guest posts from our friends in industry and academia.

Please feel free to leave comments, feedback, and questions on our posts.  We’ll try to respond in a timely manner.

– Chris