March 15, 2019  |  FlowSOM, viSNE

Beginner’s guide to FlowSOM: Profiling the innate immune response to viral infection

FlowSOM detailChikungunya virus is transmitted to humans in tropical regions through the bite of a mosquito carrying the virus. Pediatric populations are most at risk for developing acute illness of fever, rash, and joint pain, often followed chronic, debilitating arthritis. With no available vaccine or effective anti-viral treatment, a comprehensive immunophenotyping study was performed by Michlmayr et. al. on PBMCs of 42 children during acute and convalescent phases of infection, to better understand the innate anti-viral immune response.

Using 35-parameter CyTOF data available on ImmPort (SDY1288), we applied Cytobank’s machine learning tools viSNE followed by FlowSOM to simplify visualization, then cluster cells and identify which clusters were altered during acute infection. More »

November 30, 2018  |  Announcements, FlowSOM, Release Notes

Announcing: Cytobank 7.0 with FlowSOM

We are delighted to introduce our latest version of Cytobank.

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Included in this release are many performance and interface improvements, as well as a notable new addition to our repertoire of integrated algorithms. As we continue to evolve and improve our platform in response to your needs and feedback, we also look to leaders in our field to expand our capabilities and bring high-level developments in bioinformatics to you in an approachable and easy-to-use way.
In this spirit, we introduce
FlowSOM in Cytobank More »

Free Webinar Nov 28th:
Intro to FlowSOM in Cytobank with Sofie van Gassen

Free FlowSOM Webinar Wednesday, November 28, 2018 SofieCircle
9:00 AM PST / 12:00 PM EST / 6:00 PM CET

FlowSOM in Cytobank is coming soon!

Sign up now to learn from our scientists and our special guest expert for our next live webinar.

FlowSOMNovember2018When it is released to all users on Cytobank later this month, the FlowSOM algorithm will speed time to analysis and quality of clustering with self-organizing maps (SOMs) that can reveal how all markers are behaving on all cells, and can detect subsets that might otherwise be missed. If you have a large study with many events per sample or many samples, FlowSOM enables you to include all your data in the analysis, instead of restrictively subsampling. Leveraging the power of the Cytobank cloud, users can scale their compute to run up to 90 million events through FlowSOM in only 3.5 hours More »