Posts filed under ‘Flow Cytometry’
Never Email FCS Files Again!
Are you working with a collaborator who needs to see your raw data? Are you looking for help from a Cytobank administrator relating to experiment analysis?
Don’t bother opening your email client, searching for an email address, and digging through folders for your flow files. Instead, use the easy sharing features built into Cytobank. Once you have uploaded files to your account, they can be easily shared with others from within the Cytobank interface.
As always, your experiment is visible only to you until you actively choose to give permission to another user to see it. When you do choose to share an experiment, follow these easy steps:
Working with Accuri Data
Cytobank users have uploaded and analyzed data collected from more than 30 different flow cytometer models, so chances are that Cytobank can handle your data! In a recent post, we featured the ability of Cytobank to facilitate the mining of data from large datasets generated by the DVS Sciences CyTOF. This time, we will walk you through analysis of data collected on the Accuri cytometers using their CFlow software.
Image plots from Accuri CFlow
Accuri provided us with a set of sample files demonstrating the collection of data from cells stained with a PE-anti-CD4 antibody, and we’ll use this as an example. You can see from their CFlow software analysis that they achieve separation of and gate on the lymphocyte population (P1, first panel), and further separate CD4+ from CD4- cells (second two panels). We’ll show you how to do the same in Cytobank!
SPADE: Coming Soon to Cytobank!
SPADE analysis will be available through Cytobank soon. Do you want to make SPADE trees using your own fluorescence or mass cytometry data? Sign up for our newsletter or subscribe to our blog to get the latest news about when we will start to make SPADE available to our users.
Register as a user on Cytobank to be added to our mailing list. Scroll down on this page to see options for email or RSS subscriptions to our blog (bottom of the right sidebar).
Mass Cytometry: Vaporizing Cells in the Name of Science
Mass cytometry, a technique developed by DVS Sciences, represents a revolutionary spin on classic fluorescence-based flow cytometry. Instead of using antibodies tagged with fluorophores (in which spectral overlap quickly limits the number of parameters available for simultaneous detection), mass cytometry relies on antibodies tagged with transition element isotopes. Antibody-bound cells are vaporized, ionized, and analyzed on a mass spectrometer.
Cytometry in the Cloud
At Cytobank, we do cytometry in the “cloud”. What does that mean and how can that help you?
- Surviving the Data deluge
- Clarity, Access, and Collaboration
- Security and Back-up
