April 5, 2011  |  Cytobank

That Funny Looking Black-and-White Box

You may have noticed a little black-and-white box in the top right corner of your printed Illustrations. This box is a two-dimensional barcode called a QR code.

The QR code for every printed Illustration from Cytobank encodes:

  • Name of Experiment
  • Name of Illustration
  • Link to Illustration (print view)

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February 25, 2011  |  Cytobank, Education, Flow Cytometry

Immunophenotyping Tumor-Infiltrating T cells

Dataset #4659: Testing Set – T Cell Immunophenotype (trimmed)

Quantifying the percentage of cells expressing a protein of interest is a frequent goal in both basic research and clinical studies. Paired with per-cell comparisons of the level of protein expression, this approach provides a powerful way to track and immunophenotype populations of cells present in a particular sample.

One widely recognized application of flow cytometric immunophenotyping is determining the percentage of CD4+ cells in a gated lymphocyte population in order to determine prognosis for an HIV patient. Other applications include measuring a series of markers in order to distinguish between different forms of leukemia.

In Cytobank, you can use the “percent in gate” statistic to measure and display the percentage of cells in a selected gate as compared to each active population in your figure. To illustrate with a simple example, let’s examine a sample dataset looking at the percentage of CD25+ cells in a CD3+ T cell population.

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February 23, 2011  |  Cytobank

Working Towards a Faster Cytobank

In the past few months, the Cytobank Development Team has made numerous backend changes with the aim of improving performance speeds. We are happy to report that, as a result, analysis on Cytobank has sped up significantly.

Depending on your specific datasets, time to get to your results may be up to 10x faster!

What has changed?

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January 25, 2011  |  Cytobank

Are Your Data Safe on Cytobank?

Seeing the words “internet” and “experiment data” in the same sentence might give you pause to wonder, “Are my data safe on Cytobank?”

The answer is a resounding “Yes!”

From the minute you upload your data to Cytobank, you control the level of access that other users have to your data. By default, any data you upload are only visible to you.

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December 21, 2010  |  Cytobank, Flow Cytometry

Working with Compensation in Cytobank

Let’s just say it: compensation is a pain. Unfortunately, it’s a necessary pain if we want to accurately interpret our flow data and draw meaningful biological conclusions.

As a brief reminder of why we need to care about compensation, let’s examine the following emission spectra. We used the BD Fluorescence Spectrum Viewer; the Fluorescence SpectraViewer by Invitrogen/Life Technologies is also useful.

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December 20, 2010  |  Cytobank

Cytobank is on YouTube!

Did you know we have our very own YouTube channel? Our tutorial videos are designed to help you get started with using Cytobank to manage, analyze, and share your flow cytometry data.

If you’re new to Cytobank, the Cytobank Overview is a good place to start.

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December 14, 2010  |  Cytobank, Education, Flow Cytometry

Biochemistry at the Single Cell Level

Dataset #811: 090902-PBMC stim with IL6, IL10, LPS (Public Version)

Western blots are great. That is, unless you’re studying signal transduction in a mixed population of primary cells. The amount of material necessary for many biochemical techniques is usually not practical when working with patient samples or rare cell populations.

Flow cytometry to the rescue! Phospho-flow tracks biochemistry at the single cell level. Simultaneous assessment of cell surface markers and signaling events for individual cells enable biologists to profile disease samples or monitor the effects of drugs in clinical samples. More »