Seeing the words “internet” and “experiment data” in the same sentence might give you pause to wonder, “Are my data safe on Cytobank?”
The answer is a resounding “Yes!”
From the minute you upload your data to Cytobank, you control the level of access that other users have to your data. By default, any data you upload are only visible to you.
So you’ve signed up for Cytobank and you’re logged in for the first time. Now what? Here are 5 terms to learn when starting to use Cytobank.
Let’s just say it: compensation is a pain. Unfortunately, it’s a necessary pain if we want to accurately interpret our flow data and draw meaningful biological conclusions.
As a brief reminder of why we need to care about compensation, let’s examine the following emission spectra. We used the BD Fluorescence Spectrum Viewer; the Fluorescence SpectraViewer by Invitrogen/Life Technologies is also useful.
Did you know we have our very own YouTube channel? Our tutorial videos are designed to help you get started with using Cytobank to manage, analyze, and share your flow cytometry data.
If you’re new to Cytobank, the Cytobank Overview is a good place to start.
Dataset #811: 090902-PBMC stim with IL6, IL10, LPS (Public Version)
Western blots are great. That is, unless you’re studying signal transduction in a mixed population of primary cells. The amount of material necessary for many biochemical techniques is usually not practical when working with patient samples or rare cell populations.
Flow cytometry to the rescue! Phospho-flow tracks biochemistry at the single cell level. Simultaneous assessment of cell surface markers and signaling events for individual cells enable biologists to profile disease samples or monitor the effects of drugs in clinical samples. More »
Dataset #310: Phospho-Flow Cytokine Titration
Q: I want a big picture view of my flow cytometry experiment so that I can see how all of my samples are behaving under different conditions. But I also want to see the underlying single cell details because subsets of cells in these samples may be responding differently. Is this possible?
A: Absolutely. You can have your cake and eat it too by using a built-in feature on Cytobank to view-through a heatmap representation of your data. More »
Dataset #61: Welcome to Cytobank – U937 dataset
So you want to do phospho-flow? Take the U937 challenge!
What is phospho-flow? It’s a specialized form of flow cytometry that enables biologists to probe signaling activity inside the cell. Flow cytometry has been widely used to analyze cell populations by probing the proteins that are outside on the cell surface. Phospho-flow cytometry includes a few extra steps – stimulation, fixation, and permeabilization – that enable researchers to also probe the transient phosphorylation state of intracellular signaling proteins. More »
In the beginning, we wanted software that would give us a “big picture” view of our flow cytometry experiments. We initially created a software tool to make heatmaps using flow cytometry files. However, as we shared analyses with each other, we realized that this big picture view wasn’t enough. We also needed to explore the single cell data to derive deeper meaning from our flow cytometry experiments. In short, we wanted to have it both ways: we needed dynamic summaries of experiment results linked to the underlying single cell data, so that rare cell subsets and heterogeneous populations were not overlooked. More »