November 18, 2010  |  Cytobank

View Through a Heatmap: Have Your Cake and Eat It Too

Dataset #310: Phospho-Flow Cytokine Titration

Q: I want a big picture view of my flow cytometry experiment so that I can see how all of my samples are behaving under different conditions. But I also want to see the underlying single cell details because subsets of cells in these samples may be responding differently. Is this possible?

A: Absolutely. You can have your cake and eat it too by using a built-in feature on Cytobank to view-through a heatmap representation of your data. More »

November 17, 2010  |  Cytobank, Flow Cytometry

Take the U937 Challenge!

Dataset #61: Welcome to Cytobank – U937 dataset

So you want to do phospho-flow? Take the U937 challenge!

What is phospho-flow? It’s a specialized form of flow cytometry that enables biologists to probe signaling activity inside the cell. Flow cytometry has been widely used to analyze cell populations by probing the proteins that are outside on the cell surface. Phospho-flow cytometry includes a few extra steps – stimulation, fixation, and permeabilization – that enable researchers to also probe the transient phosphorylation state of intracellular signaling proteins. More »

October 14, 2010  |  Cytobank

The Cytobank Story

In the beginning, we wanted software that would give us a “big picture” view of our flow cytometry experiments.  We initially created a software tool to make heatmaps using flow cytometry files.  However, as we shared analyses with each other, we realized that this big picture view wasn’t enough.  We also needed to explore the single cell data to derive deeper meaning from our flow cytometry experiments.  In short, we wanted to have it both ways: we needed dynamic summaries of experiment results linked to the underlying single cell data, so that rare cell subsets and heterogeneous populations were not overlooked. More »