Ever find yourself staring at a folder of FCS files and thinking, “Wait, now which tubes did I add PMA to, how much did I add, and which samples were these again?”
Jonathan from Cytobank/Stanford recommends what he calls “future proofing” in order to avoid this problem. He explained this approach during a CYTO 2011 Pre-Congress course in his talk titled “Flood Cytometry: Embracing Single Cell Systems Biology (and coping with large cytometry experiments).” In that talk, he outlined four easy steps that are useful for experiments of all sizes.
When collecting on the cytometer:
- Tag your FCS files with key experiment details (e.g. “Patient-J01 IL-2 15m”)
- Label the channels you are measuring (before collecting data)
- Make sure scales and compensations work (before collecting data)
- Encode clinical sample IDs (don’t use HIPAA sensitive information)
Because Cytobank is web-based, we can help you share your flow data with your advisor, labmates, and collaborators – all around the world! By default, your data is private and visible only to you, but if you do decide to share, the easiest way to share an experiment is to give another user full access. But what if you need to share dozens of experiments with the same three people? Or what if you don’t want to give full access to someone but still want them to see your Illustrations?
A Cytobank project enables you to share multiple experiments with the same group of people. A project also allows you to set project-wide permission levels that let you determine whether or not people can make new illustrations and whether or not they can clone the experiments contained within the project.
Our summer travels are not quite over! Come say hello and tell us how you’re using Cytobank to share and analyze your flow data.
We’re coming to a city near you. Come say hello and tell us how you’re using Cytobank to share and analyze your flow data.
We’re excited to be attending both IMMUNOLOGY 2011 and CYTO 2011 in the next couple weeks. Come say hello and tell us how you’re using Cytobank to share and analyze your flow data.
May 13-17, 2011
98th Annual Meeting of the American Association of Immunologists
Moscone Center, San Francisco, CA
May 21-25, 2011
XXVI Congress of the International Society for Advancement of Cytometry (ISAC)
Baltimore Convention Center, Baltimore, MD
Mass cytometry, a technique developed by DVS Sciences, represents a revolutionary spin on classic fluorescence-based flow cytometry. Instead of using antibodies tagged with fluorophores (in which spectral overlap quickly limits the number of parameters available for simultaneous detection), mass cytometry relies on antibodies tagged with transition element isotopes. Antibody-bound cells are vaporized, ionized, and analyzed on a mass spectrometer.