February 28, 2014  |  User Stories

Cytobank User Stories: Olga Ksionda, Ph.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of fluorescence and mass cytometry. This time we interview Olga Ksionda, Ph.D., a postdoctoral scholar in the lab of Jeroen Roose in the Department of Anatomy at the UCSF School of Medicine. Send us feedback and let us know who you’d like to hear from (including yourself)!

What are you excited about in science? What is your scientific vision?
Olga Ksionda, Ph.D.
Olga Ksionda, Ph.D.
UCSF School of Medicine

I am very much interested in understanding the molecular aspects of T cell development, especially in pathological settings when wrong decisions are “made” and T cells become autoimmune or malignant.

In general terms, I have always found it fascinating when unrelated fields cross fertilize with unexpected outcomes. I consider some of the best examples of this the Cre system or recently described CRISPR technology.

More »

January 23, 2014  |  User Stories

Cytobank User Stories: Hervé Luche, Ph.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of fluorescence and mass cytometry.

This time we interview Hervé Luche, Ph.D., R&D Manager at the Centre d’Immunophénomique in Marseilles, France.

Send us feedback and let us know who you’d like to hear from (including yourself)!

What are you excited about in science? What is your scientific vision?
Herve Luche,
Herve Luche, Ph.D.

I am very much interested in trying to understand how cells integrate signals and communicate with their environment to actually change their transcriptional programs, fate or acquire a new function. To try to tackle down this complexity in biology, one necessarily needs multi-disciplinary approaches and to combine multiple-expertise. I like sharing ideas with other people of different scientific backgrounds to establish new models and theories. The most exciting part of the work to me is when you bring all pieces of the puzzle together and that your model becomes partly true and clarifies one scientific question. It does not happen very often (to me at least!) but when it does, it is great fun!
More »

January 21, 2014  |  Announcements

Cytobank 3.0: Enhanced gating, upload, and download

Cytobank 3.0 marks a new chapter in our journey where users can now enjoy a streamlined upload, download, and gating experience. We’d like to take this opportunity to introduce you to what’s new:

Uploading and Downloading

Data upload and download have a new interface, and the old Java applets have been retired. This means faster uploading and downloading and fewer compatibility issues.

We’ve also added mechanisms for users to upload ACS and ZIP files, and have expanded the prevalence and detail-level of progress bars. Want to easily transfer experiments between your accounts on different Cytobank servers? Users can now export ACS versions of their experiments from the Experiment Details page and use ACS upload to move them over.

Enhanced Gating Interface

Like upload and download, gating has received a makeover and no longer uses Java. The first thing you’ll notice is improved loading time and fewer compatibility issues. The new interface also includes several frequently-requested enhancements, including axis tick marks, display of gate percentages, marking of tailored gates (T), marking of hidden gates (H), display of both channel short names and long names, and an expandable plot scales section. More »

January 14, 2014  |  Announcements

Reflecting on this past year’s journey of growth at Cytobank

As the year draws to a close, we’re reflecting on this past year’s journey of growth at Cytobank.

We’re excited to have welcomed thousands of new users from around the world to our community in 2013, representing a diversity of users from academia and industry. Many of these users contributed to our growing list of Publications Referencing Cytobank. We’ve now processed over a million FCS files on our Community Edition alone and anticipate even more in the coming year.  This year, we also increased our capacity including addition of a European datacenter to accommodate the needs of our customers across the pond.

Trends in the field highlight the importance of single cell measurements, and we are hard at work to make sure we can enable your single cell analysis endeavors. Developing and partnering to bring you the latest in single cell analysis is high on our priority list, for cytometry and beyond.

We’ve kept our ears attuned to your feedback and launched nine major releases that improve Cytobank based on common user requests. We released a visual plate annotator, a module for enhanced export of customized statistics, extensive SPADE upgrades, expanded options for uploading and downloading (ZIP and ACS), and speed enhancements, to name a few. We also rolled out Cytobank 3.0, which features a redesigned, HTML5 gating interface as well as faster times for data transfer.   More »

March 29, 2013  |  Education

New Fluorescence and Mass Cytometry Protocols

One of our goals at Cytobank is to build community resources that facilitate cytometry experiments. This time around, we’ve updated a couple of our Resources with protocol sheets. These protocol sheets contain experimental steps linked to analysis data that you work with yourself in Cytobank. If you’re just venturing out into the realm of mass cytometry, or phospho-flow using mass or fluorescence cytometry, these resources can guide you along the way.
More »

February 28, 2013  |  User Stories

Cytobank User Stories: Eric Padron, M.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Eric Padron, M.D., Clinical Instructor at the H. Lee Moffitt Cancer Center. Dr. Padron is in the laboratory of PK Epling-Burnette in the division of Malignant Hematology.

Send us feedback and let us know who you’d like to hear from (including yourself)!

What are you excited about in science? What is your scientific vision?
Eric Padron M.D. - Moffitt Cancer Center
Eric Padron M.D. – Moffitt Cancer Center

The genomic age has given investigators an unprecedented window in to the molecular abnormalities within cancer cells. Through this window, we see that there are many molecular abnormalities, some occurring in divergent pathways. As a physician-scientist I am most interested in identifying which of these pathways are required for cancer cell viability/proliferation and leveraging this information for the design of genomically targeted pharmaceuticals in clinical trials. More »