May 20, 2011  |  Announcements, Flow Cytometry

What is SPADE?

What is SPADE?

SPADE (Spanning-tree Progression Analysis of Density-normalized Events) is a way to automatically identify populations in multidimensional flow cytometry data files. SPADE clusters cells into populations and then projects them into a tree like the one shown below. SPADE works for data from both ‘classic’ fluorescence flow cytometry and mass cytometry.

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Webinar Recording: Explore Hidden Cell Populations with cytoclustR

TregsviSNEShahramOur recent Webinar with Dr. Shahram Kordasti and Bioinformatician Thanos Mourikis explored cytoclustR, a new tool they developed with the Cytobank API.

Dr. Kordasti began by reviewing how he set up his experiment pipeline to best identify Treg populations, as outlined in his article featured on the cover of Blood Journal.

Thanos followed with a step-by-step tutorial on how to use cytoclustR with SpadeTreeShahramSPADE in Cytobank to distinguish and report on hidden cell populations that might otherwise go unnoticed with manual gating strategies.

cyobankarrow40x40Check out the recording on our YouTube channel

July 18, 2018  |  Announcements

Manage Experiments More Efficiently with Cytobank 6.3.1

storagedetailCytobank’s latest release introduces functionality to help you better manage your account storage and more easily clone experiment features

Improved Storage Management
You can now see how much storage space you are using, and can filter by experiment size in the inbox. Understand quickly which of your experiments may be more resource-intensive, cull unused or duplicate experiments, and keep your storage usage in check.

Improvements to cloning and sharing (learn more)
Select which components from your clonedetail-2experiments to clone. Carry over sharing permissions, compensation matrices separately from gates, panels separately from sample tags, and more. You can also choose whether to give your PI full access when you clone experiments. These features are also fully-supported in the Cytobank API.

Iterate and Refine Faster 
You can now quickly cancel SPADE, viSNE, and CITRUS runs in progress to correct mistakes or refine settings.

 

June 28, 2018  |  API, Education, SPADE, viSNE, Webinars

July Webinar:
Explore Hidden Cell Populations with cytoClustR and the Cytobank API

Best Practices for Adopting Clustering Pipelines in Place ofSPADE Tree Kordasti Manual Gating

Join us Wednesday, July 18 at 9:00 AM PDT for our next free webinar. Learn how Physician Scientist, Dr. Shahram Kordasti of King’s College London and Bioinformatician Thanos Mourikis of Cancer Research UK (CRUK) collaborated to build cytoClustR, a tool you can use with Cytobank’s machine learning algorithms to identify cell subsets in order to help make discoveries in the clinic.

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May 25, 2018  |  Education, Events, viSNE, Webinars

Webinar Recap: Advanced viSNE (t-SNE) Review

Untitled-1Nearly 500 scientists registered for our latest data analysis webinar. Cytobank Application Scientist Geoff Kraker took us on a tour of recently added features like setting the seed (for replicating results), configuring display gate labels, and more. If you missed it, not to worry. The recording is now available online.

cyobankarrow40x40View our latest webinars on the Cytobank YouTube channel

Following the demo, Geoff fielded many great live questions from the viewers. Here are some of our favorites: More »

April 11, 2018  |  Announcements, Conferences, Flow Cytometry

Announcing Cytobank’s Collaboration with Cytek Biosciences

We’rCytekLogoe excited to announce a new collaboration with Cytek, makers of the innovative CytekTM Aurora Flow Cytometer. Cytek’s Aurora and Cytobank’s next-generation analytics platform have united in their goal to make high-dimensional data and its subsequent machine learning analysis more accessible to more scientists.

  • Aurora’s unique optical design combined with spectral unmixing delivers quality, high-dimensional data where rare and dim populations can be easily resolved, regardless of sample complexity.
  • Cytobank’s algorithms simplify visualization and analysis of high-dimensional unmixed data and allow you to quickly share discrete insights from these data.

Here are two specific examples of how you can apply our combined solutions to enable faster, more highly-resolved discoveries: More »

March 13, 2018  |  Announcements, API, Education

Introducing Cytobank BridgeR: An easy-to-use R package for Biologists

imagesThe Cytobank API opens up our platform to workflows and creative use beyond native functionality to improve the efficiency and velocity of your research efforts. We are excited to enhance the usability of the Cytobank API with the release of BridgeR, an easy-to-use R package for biologists.

Experience working in R isn’t required.  Our step-by-step guide provides instructions on how to execute the API-leveraged workflows detailed in BridgeR. More »