March 12, 2015  |  Announcements, Education  |  By  |  0 Comments

Complete your viSNE workflow and back-gate using Dot Overlays

Always listening to our user feedback, we heard your requests to complete your viSNE workflow by overlaying Populations to uniquely color the viSNE continents. Our latest release brings you a new plot type configuration we call Dot Overlays that allows you to do this, and also lends itself to traditional back-gating approaches that allow you to see child populations in the context of ancestors.

Dot Overlays
Dot Overlays

Dot Overlays allow you to overlay not just Populations, but any of the Figure Dimensions within a single Dot Plot. Now you can compare Populations, Timepoints, Conditions, Individuals, and any dimension of your choosing within Dot Overlay plots. Just drag any figure dimension into the second (overlaid) position. See how your time course stacks out, compare your stimulations/titration data, or just see how populations sit in the context of each other (traditional back-gating). Visit our knowledge base for the full scoop on how to set up Dot Overlays, including a video tutorial.

Dot Overlays - viSNE
Dot Overlays – viSNE

Dot Overlays allow you to see your viSNE continents come to life as they are colored by the variables of your choosing. One common workflow is to manually gate Populations and overlay them on the viSNE continent map to see how manually gated data compares to the automated viSNE approach. This can be another way to cross-check which populations are which, in addition to using the ability to color by Channel.

Histogram Overlay - Color by Row
Histogram Overlay – Color by Row

Related to Dot Overlays, we’ve also implemented a long-standing user request to be able to uniquely color overlaid histogram variables. This allows you to more rapidly distinguish overlaid variables, while our Cytobank Classic Histogram Overlays (with Statistic Fill) are still available and show you an intuitive statistics-based heat coloring.