April 7, 2014  |  User Stories  |  By  |  0 Comments

Cytobank User Stories: Kanutte Huse, Ph.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of fluorescence and mass cytometry.

This time we interview Kanutte Huse, Ph.D., a postdoctoral fellow at the Institute for Cancer Research, Oslo University Hospital, Norway. Currently, Kanutte is a visiting research fellow in the Irish lab at Vanderbilt University.

Send us feedback and let us know who you’d like to hear from (including yourself)!

What are you excited about in science? What is your scientific vision?
Kanutte Huse,
Kanutte Huse,
 Ph.D. Postdoctoral Fellow at Oslo University Hospital

The short answer is everything. I’m impressed by how natural scientists can measure and model everything around us, continuously increasing our knowledge about the world. More specifically, I’m interested in understanding the signaling networks of cells, and it fascinates me how seemingly simple signaling pathways can regulate a range of functional outcomes. I want to understand how alterations in signaling pathways lead to cancer.

What do you study / what is your field?
I study cancer in the context of immunology. My field is B cells and B-cell lymphoma, and I study signaling in these cells. During my Ph.D., I studied the role of TGF-β ligands in normal and healthy B cells [1, 2], and now I focus on B cell receptor signaling.

What do you use flow cytometry for?
I use phospho-flow to study B cell signaling and I also use flow cytometry to measure functional outcomes like apoptosis, differentiation and proliferation in response to cytokines.  Recently, I have started using CyTOF mass cytometry for high dimensional immunophenotyping.

What are some of your favorite papers?
The work I do now is primarily inspired by two papers, both using phospho-flow to study signaling in B cells. One is by Khalil et al. [3], showing that BCR signaling is reduced in germinal center B cells due to phosphatase activity. The other is by Irish et al. [4], showing the importance of BCR signaling in follicular lymphoma.

What do you do for fun?
I enjoy outdoor activities like rock climbing, hiking and skiing, especially when I can do this together with my husband and two kids.

What’s your favorite thing about Cytobank?
The best thing about Cytobank is that it’s available from anywhere and that it’s easy to share data with colleagues and collaborators. I also like the way it displays data and how easy it is to rearrange figures.

1. Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7. Huse K et al. Eur J Immunol (2011) 41(11):3135-45. [PubMed]

2. Role of Smad Proteins in Resistance to BMP-Induced Growth Inhibition in B-Cell Lymphoma. Huse K et al. PLoS ONE (2012). [Journal]

3. B Cell Signal Transduction in Germinal Center B Cells is Short-Circuited by Increased Phosphatase Activity. Khalil AM et al. Science (2012) 336(6085): 1178–1181. [Journal]

4. B-cell signaling networks reveal a negative prognostic human lymphoma cell subset that emerges during tumor progression. Irish JM et al. PNAS (2010) 107(29):12747–12754. [Journal]

Interview conducted by Cytobank staff member Angela Landrigan.