September 30, 2013  |  User Stories  |  By  |  0 Comments

Cytobank User Stories: Emilie Decaup

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow and mass cytometry.

This time we interview Emilie Decaup, a Ph.D. Student at Toulouse University whose work on follicular lymphoma was recently published in the Journal of Blood Cancer.

Send us feedback and let us know who you’d like to hear from (including yourself)!

What are you excited about in science? What is your scientific vision?
Emilie Decaup - Ph.D. Student at Toulouse University
Emilie Decaup – Ph.D. Student at Toulouse University

I am really excited by cancer research. There is so much to do and to discover particularly for therapy. It is a domain in perpetual evolution with ever new therapeutic targets and drugs. Technological advances in biomedical research allow us to learn more. Flow cytometry is a technique that I particularly appreciate because it is really powerful especially for studying mechanisms of action of therapeutic agents like monoclonal antibodies. New technical approaches like fluorescent cell barcoding simplify experiments with lot of samples and allow straightforward drug screening. I think that technological advances will allow researchers to be more efficient.

What do you study / what is your field?
I am studying the intracellular signals induced by anti-CD20 monoclonal antibodies in a model of 3D culture of follicular lymphoma cells.
What do you use flow cytometry for?
I use flow for several things. First I use it for analyzing cell biology: phenotyping, cell cycle, cell death or also proliferation. Then I also use it for studying intracellular signaling pathways. For example I used phosphoflow and fluorescent cell barcoding to determine effects of monoclonal antibodies on classical signaling pathways known to be overactivated in follicular lymphoma.
What are some of your favorite papers?
I will cite two papers. First the one published in Nature Methods by Krutzik PO and Nolan G entitled “Fluorescent cell barcoding in flow cytometry allows high-throughput drug screening and signaling profiling” because it makes me discover this method that I used for my project. Then I want to cite the review article entitled “Monoclonal antibody therapy for B cell non-Hodgkin’s lymphoma” published in 2008 by Cheson and Leonard. Even if there is a lot of upgrades in this domain, I think that is a reference, really well written and well illustrated.
What do you do for fun?
Horse riding for pleasure and in competition.
What’s your favorite thing about Cytobank?
It permits to analyze quickly a very large number of samples. The analysis is quite simple thanks to Cytobank didactics of. Furthermore, we can work on our data both at the office and at home, which is really useful.