Nolan Lab Signaling-Based Cytometry Resource
As part of educational and community outreach efforts in flow cytometry, the Nolan Lab at Stanford University has worked with the Cytobank team to launch a Signaling-Based (Fluorescence & Mass) Cytometry Resource. The Nolan lab has authored several key publications in flow cytometry on subjects including phosphoflow cytometry, barcoding, mass cytometry, and SPADE. Visit this Cytobank-powered resource to find experiment protocols for fluorescence and mass cytometry, Cytobank Reports, and Publications.
The Experiment Protocols section of the resource links to protocols for fluorescence and mass cytometry, including those relevant to stimulations, barcoding, fix/perm methods, and titrating antibodies.
Here you’ll find protocols related to metal labeling of IgG antibodies for use in mass cytometry experiments.
We’ve prepared interactive reports of published Nolan Lab datasets. Visit the Reports section of the resource to view and actively analyze their published datasets first-hand. Viewing Reports can help you whether you’re interested in the Nolan Lab’s datasets in more detail, wanting to learn to use Cytobank in a guided way or looking to get a feel for how mass cytometry datasets are analyzed on Cytobank.
Future Directions of the Resource
Check back as we update the resource to include tutorials and a FAQ section. We hope you’ll find this resource to be helpful to your fluorescence and mass cytometry endeavors! We are just getting started with the resource and will be adding more content over time. Subscribe to the Cytobank newsletter for information on updates.