July 30, 2012  |  User Stories  |  By  |  0 Comments

Cytobank User Stories: Greg Behbehani, Ph.D., M.D.

Welcome to Cytobank User Stories, a series featuring interviews with Cytobank users on their research, scientific vision, and use of flow cytometry.

This time we interview Greg Behbehani, Ph.D., M.D., Postdoctoral Fellow in the Nolan Lab, affiliated with the Baxter Laboratory of Stem Cell Biology and the Departments of Hematology and Oncology at Stanford University School of Medicine. Greg’s recent publications include his work developing cell cycle analysis for mass cytometry using the human hematopoietic hierarchy as a model system and is featured on the cover of Cytometry this month. You can interact directly with these data via Greg’s Cytobank Report, which can also be found on the Nolan Lab Signaling-Based (Fluorescence & Mass) Cytometry Resource.

Send us feedback and let us know who you’d like to hear from (including yourself)!

What are you excited about in science? What is your scientific vision?
Greg Behbehani, Ph.D., M.D., Postdoctoral Fellow, Nolan Lab, Stanford University

My research is focused on understanding how human cancer cells grow and die, specifically in response to chemotherapy treatments.  I’m excited about the new possibilities that have been opened up in systems biology.  Ideally, I would like to design experiments to test some of the hypotheses that have been advanced regarding how cancer cell populations become resistant to chemotherapy using primary cell populations and analyzing responses across all of the different cancer cell sub-populations.  While these types of experiments have been tried before, I think we now have the technologies that might allow us to be successful at using these techniques to predict chemotherapy responses.

What do you study / what is your field?
I do translational research aimed at understanding cancer growth and how cancer cells respond to chemotherapy.
What do you use flow cytometry for?
I focus on mass cytometry and flow cytometry techniques, which I feel are best suited to understanding the multiple small subpopulations that are resistant to treatment and lead to cancer recurrence.
What are some of your favorite papers?
I like “big” science.  I knew that I wanted to come to Stanford when I presented the first gene expression microarray paper from Patrick Brown’s lab.  I just like to take a more open-ended approach to science.Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Schena M, Shalon D, Davis RW, Brown PO. Science. (1995) 270(5235):467-70.
What do you do for fun?
My two-year-old keeps me pretty busy outside of the lab, but when I have time I love to hike, bike, and run.
What’s your favorite thing about Cytobank?
Doing 40 parameter experiments, I generate a lot of data and Cytobank lets me look at all of it at once and easily compare individual channels across all of the conditions and populations in my experiment.  I also love the ability to rearrange the relationships between gates and sub-gates at the click of a button.

Interview conducted and presented by Cytobank staff member Angela Landrigan.