What is SPADE?

May 20, 2011 at 4:56 pm 1 comment

What is SPADE?

SPADE (Spanning-tree Progression Analysis of Density-normalized Events) is a way to automatically identify populations in multidimensional flow cytometry data files. SPADE clusters cells into populations and then projects them into a tree like the one shown below. SPADE works for data from both ‘classic’ fluorescence flow cytometry and mass cytometry.

The SPADE tree above shows CD14 expression in different cell populations in human bone marrow. To read more about SPADE, look up the mass cytometry study recently published in Science from Garry Nolan’s lab at Stanford and DVS Sciences, now a Fluidigm Company.

– The Cytobank Team


Bendall SC, Simonds EF, Qiu P, Amir ED, Krutzik PO, Finck R, Bruggner RV, Melamed R, Trejo A, Ornatsky OI, Balderas RS, Plevritis SK, Sachs K, Pe’er D, Tanner SD, Nolan GP. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011 May 6; 332(6030):687-96. Abstract.

Entry filed under: Announcements, Flow Cytometry. Tags: , , , , , , .

Upcoming Meetings: IMMUNOLOGY 2011 and CYTO 2011 Working with Accuri Data

1 Comment Add your own

  • 1. Nora Sarvetnick  |  November 14, 2011 at 3:47 pm

    when will SPADE be available?


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