SPADE: Coming Soon to Cytobank!
SPADE analysis will be available through Cytobank soon. Do you want to make SPADE trees using your own fluorescence or mass cytometry data? Sign up for our newsletter or subscribe to our blog to get the latest news about when we will start to make SPADE available to our users.
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What is SPADE?
SPADE (which stands for spanning-tree progression analysis of density-normalized events) is a way to automatically identify populations in multidimensional flow cytometry data files. SPADE clusters cells into populations and then projects them into a tree like the one shown above. SPADE works for data from both ‘classic’ fluorescence flow cytometry and mass cytometry.
The SPADE tree above shows CD14 expression in different cell populations in human bone marrow. To read more about SPADE, look up the mass cytometry study recently published in Science from Garry Nolan’s lab at Stanford and DVS Sciences.
- The Cytobank Team
Bendall SC, Simonds EF, Qiu P, Amir ED, Krutzik PO, Finck R, Bruggner RV, Melamed R, Trejo A, Ornatsky OI, Balderas RS, Plevritis SK, Sachs K, Pe’er D, Tanner SD, Nolan GP. Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum. Science. 2011 May 6; 332(6030):687-96. Abstract.