January 17, 2011  |  Announcements  |  By  |  1 Comment

5 Terms You Need to Know in Cytobank

So you’ve signed up for Cytobank and you’re logged in for the first time. Now what? Here are 5 terms to learn when starting to use Cytobank.

1. Inbox

The Inbox is the first page to which you are directed upon signing in to Cytobank. It contains a list of your uploaded experiments, experiments other users have shared with you, and public experiments. Much like an email inbox, you can tag experiments with labels for filtering and use the search box to further filter results. Clicking on an experiment name will open the experiment, at which point you can proceed with analysis inside it.

2. Illustrations

An Illustration contains the data plots associated with your experiment. You build Illustrations dynamically on an experiment’s Working Illustration page using Sample Tags belonging to the experiment.

3. Sample Tags

Every scientific experiment has variables that are being tested. Annotating your data with Sample Tags is how you bring the scientific information about an experiment into Cytobank. Once this information is present, it can be used to drive the analysis process, allowing you to work at the level of named variables instead of individual FCS files. Cytobank has features to automatically annotate FCS files based on information in the file.

4. Figure Dimensions

Figure Dimensions are boxes within the Working Illustration that are used to drive figure generation. The information from Sample Tags present in the experiment appears in Figure Dimensions boxes, allowing you to toggle parts of the experiment on and off easily. The boxes themselves can be dragged left and right to adjust figure layout.

5. Channels and Panels

The term “Channels” refers to the flow cytometer channel on which your data were collected. If you used different detection reagents on the same channel for different samples (e.g., pERK1/2-PE for some samples and pAkt-PE for other samples), you can define staining “Panels” to group the samples accordingly. Learn more about panels and channel.

Want to learn more about Cytobank concepts? Visit the Cytobank documentation site, and in particular, our core overviews section. More questions? We’re happy to help. There are many ways to get support!