Archive for December, 2010
Working with Compensation in Cytobank
Let’s just say it: compensation is a pain. Unfortunately, it’s a necessary pain if we want to accurately interpret our flow data and draw meaningful biological conclusions.
As a brief reminder of why we need to care about compensation, let’s examine the following emission spectra. We used the BD Fluorescence Spectrum Viewer; the Fluorescence SpectraViewer by Invitrogen/Life Technologies is also useful.
December 21, 2010 at 9:00 am Stephanie Huang Leave a comment
Cytobank is on YouTube!
Did you know we have our very own YouTube channel? Our tutorial videos are designed to help you get started with using Cytobank to manage, analyze, and share your flow cytometry data.
If you’re new to Cytobank, the Cytobank Overview is a good place to start.
December 20, 2010 at 9:00 am Stephanie Huang Leave a comment
Upcoming Meetings: December 2010 – May 2011
2010 is quickly coming to a close. We’re already planning for meetings through next spring! Hope to see some of you at the following events. Come say hello and tell us how you’re using Cytobank to share and analyze your flow data. (more…)
December 15, 2010 at 10:30 am Stephanie Huang Leave a comment
Biochemistry at the Single Cell Level
Dataset #811: 090902-PBMC stim with IL6, IL10, LPS (Public Version)
Western blots are great. That is, unless you’re studying signal transduction in a mixed population of primary cells. The amount of material necessary for many biochemical techniques is usually not practical when working with patient samples or rare cell populations.
Flow cytometry to the rescue! Phospho-flow tracks biochemistry at the single cell level. Simultaneous assessment of cell surface markers and signaling events for individual cells enable biologists to profile disease samples or monitor the effects of drugs in clinical samples. (more…)
