Archive for November, 2010

View Through a Heatmap: Have Your Cake and Eat It Too

Dataset #310: Phospho-Flow Cytokine Titration

Q: I want a big picture view of my flow cytometry experiment so that I can see how all of my samples are behaving under different conditions. But I also want to see the underlying single cell details because subsets of cells in these samples may be responding differently. Is this possible?

A: Absolutely. You can have your cake and eat it too by using a built-in feature on Cytobank to view-through a heatmap representation of your data. (more…)

November 18, 2010 at 7:52 am 1 comment

Take the U937 Challenge!

Dataset #61: Welcome to Cytobank – U937 dataset

So you want to do phospho-flow? Take the U937 challenge!

What is phospho-flow? It’s a specialized form of flow cytometry that enables biologists to probe signaling activity inside the cell. Flow cytometry has been widely used to analyze cell populations by probing the proteins that are outside on the cell surface. Phospho-flow cytometry includes a few extra steps – stimulation, fixation, and permeabilization – that enable researchers to also probe the transient phosphorylation state of intracellular signaling proteins. (more…)

November 17, 2010 at 6:06 pm 1 comment

Upcoming Meetings: November 2010 – January 2011

We’re coming to a city near you. Come say hello and tell us how you’re using Cytobank to share and analyze your flow data. (more…)

November 10, 2010 at 10:00 am Leave a comment

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